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  1. Library Resource
    Policy Papers & Briefs
    December, 2010
    Czech Republic

    The aim of this study was to verify the accuracy of the pedigree data of Old Kladruber dam lines using mtDNA analysis. The upstream part of the mtDNA D-loop hypervariable region (384 bp, nt15450 - nt15834) was sequenced. DNA was extracted from deep-frozen whole blood and fresh saliva samples of 170 Old Kladruber horses representing 23 dam lines from Kladruby n/L stud and some private farms.

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