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Land use and land cover change, perhaps the most significant anthropogenic disturbance to the environment, mainly due to rapid urbanization/industrialization and large scale agricultural activities. In this paper, an attempt has been made to appraise land use/land cover changes over a century (1914–2007) in the Neyyar River Basin (L=56 km; Area = 483.4 km²) in southern Kerala – a biodiversity hot spot in Peninsular India. In this study, digital remote sensing data of the Indian Remote Sensing satellite series I-D (LISS III, 2006–2007) on 1:50,000 scale, Survey of India (SOI) toposheet of 1914 (1:63,360) and 1967 (1:50,000) have been utilized to map various land use/land cover changes. Maps of different periods have been registered and resampled to similar geographic coordinates using ERDAS Imagine 9.0. The most notable changes include decreases in areas of paddy cultivation, mixed crops, scrub lands and evergreen forests, and increases in built-up areas, rubber plantations, dense mixed forests, and water bodies. Further, large scale exploitation of flood plain mud and river sand have reached menacing proportions leading to bank caving and cut offs at channel bends. Conservation of land and water resources forms an important aspect of ecosystem management in the basin.